Immunohistochemical mapping of neurotensin in the alpaca diencephalon

Manuel Lisardo Sánchez, Arturo Mangas, Luis Enrique Medina, Luis Angel Aguilar, Zaida Díaz-Cabiale, José Angel Narváez, Rafael Coveñas

Resultado de la investigación: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

2 Citas (Scopus)

Resumen

Introduction. The distribution of the immunoreactive cell bodies and fibers containing neurotensin in the alpaca diencephalon was determined by an immunohistochemical technique.
Material and methods. The study was carried out in four male alpacas that lived at sea level. Brains of deeply anesthetized animals were fixed by perfusion with 4% paraformaldehyde. Cryostat sections were stained by a standard immunohistochemical method.
Results. Cell bodies containing neurotensin were observed in the zona incerta and hypothalamus. A low/moderate density of these cell bodies was observed in the lateral hypothalamic area, anterior and dorsal hypothalamic areas, suprachiasmatic nucleus, periventricular region of the hypothalamus and in the ventromedial hypothalamic nucleus. In both thalamus and hypothalamus, immunoreactive fibers showed a widespread distribution. In the thalamus, a high density of these fibers was mainly found in the midline nuclei, whereas in the hypothalamus a high density was in general observed in the whole structure.
Conclusions. In comparison with other mammals, the thalamus of the alpaca showed the most widespread distribution of neurotensin-immunoreactive fibers. The widespread distribution of neurotensin through the alpaca diencephalon suggests that the peptide can be involved in many physiological actions.
Idioma originalInglés
Páginas (desde-hasta)49-58
Número de páginas10
PublicaciónFolia Histochemica et Cytobiologica
Volumen56
N.º1
DOI
EstadoPublicada - 11 abr 2018

Huella dactilar

New World Camelids
Diencephalon
Neurotensin
Hypothalamus
Thalamus
Anterior Hypothalamic Nucleus
Ventromedial Hypothalamic Nucleus
Lateral Hypothalamic Area
Suprachiasmatic Nucleus
Oceans and Seas
Mammals
Perfusion
Peptides
Brain
Cell Body

Citar esto

Sánchez, M. L., Mangas, A., Medina, L. E., Aguilar, L. A., Díaz-Cabiale, Z., Narváez, J. A., & Coveñas, R. (2018). Immunohistochemical mapping of neurotensin in the alpaca diencephalon. Folia Histochemica et Cytobiologica, 56(1), 49-58. https://doi.org/10.5603/FHC.a2018.0003
Sánchez, Manuel Lisardo ; Mangas, Arturo ; Medina, Luis Enrique ; Aguilar, Luis Angel ; Díaz-Cabiale, Zaida ; Narváez, José Angel ; Coveñas, Rafael. / Immunohistochemical mapping of neurotensin in the alpaca diencephalon. En: Folia Histochemica et Cytobiologica. 2018 ; Vol. 56, N.º 1. pp. 49-58.
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abstract = "Introduction. The distribution of the immunoreactive cell bodies and fibers containing neurotensin in the alpaca diencephalon was determined by an immunohistochemical technique. Material and methods. The study was carried out in four male alpacas that lived at sea level. Brains of deeply anesthetized animals were fixed by perfusion with 4{\%} paraformaldehyde. Cryostat sections were stained by a standard immunohistochemical method.Results. Cell bodies containing neurotensin were observed in the zona incerta and hypothalamus. A low/moderate density of these cell bodies was observed in the lateral hypothalamic area, anterior and dorsal hypothalamic areas, suprachiasmatic nucleus, periventricular region of the hypothalamus and in the ventromedial hypothalamic nucleus. In both thalamus and hypothalamus, immunoreactive fibers showed a widespread distribution. In the thalamus, a high density of these fibers was mainly found in the midline nuclei, whereas in the hypothalamus a high density was in general observed in the whole structure.Conclusions. In comparison with other mammals, the thalamus of the alpaca showed the most widespread distribution of neurotensin-immunoreactive fibers. The widespread distribution of neurotensin through the alpaca diencephalon suggests that the peptide can be involved in many physiological actions.",
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Sánchez, ML, Mangas, A, Medina, LE, Aguilar, LA, Díaz-Cabiale, Z, Narváez, JA & Coveñas, R 2018, 'Immunohistochemical mapping of neurotensin in the alpaca diencephalon', Folia Histochemica et Cytobiologica, vol. 56, n.º 1, pp. 49-58. https://doi.org/10.5603/FHC.a2018.0003

Immunohistochemical mapping of neurotensin in the alpaca diencephalon. / Sánchez, Manuel Lisardo; Mangas, Arturo; Medina, Luis Enrique; Aguilar, Luis Angel; Díaz-Cabiale, Zaida; Narváez, José Angel; Coveñas, Rafael.

En: Folia Histochemica et Cytobiologica, Vol. 56, N.º 1, 11.04.2018, p. 49-58.

Resultado de la investigación: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

TY - JOUR

T1 - Immunohistochemical mapping of neurotensin in the alpaca diencephalon

AU - Sánchez, Manuel Lisardo

AU - Mangas, Arturo

AU - Medina, Luis Enrique

AU - Aguilar, Luis Angel

AU - Díaz-Cabiale, Zaida

AU - Narváez, José Angel

AU - Coveñas, Rafael

PY - 2018/4/11

Y1 - 2018/4/11

N2 - Introduction. The distribution of the immunoreactive cell bodies and fibers containing neurotensin in the alpaca diencephalon was determined by an immunohistochemical technique. Material and methods. The study was carried out in four male alpacas that lived at sea level. Brains of deeply anesthetized animals were fixed by perfusion with 4% paraformaldehyde. Cryostat sections were stained by a standard immunohistochemical method.Results. Cell bodies containing neurotensin were observed in the zona incerta and hypothalamus. A low/moderate density of these cell bodies was observed in the lateral hypothalamic area, anterior and dorsal hypothalamic areas, suprachiasmatic nucleus, periventricular region of the hypothalamus and in the ventromedial hypothalamic nucleus. In both thalamus and hypothalamus, immunoreactive fibers showed a widespread distribution. In the thalamus, a high density of these fibers was mainly found in the midline nuclei, whereas in the hypothalamus a high density was in general observed in the whole structure.Conclusions. In comparison with other mammals, the thalamus of the alpaca showed the most widespread distribution of neurotensin-immunoreactive fibers. The widespread distribution of neurotensin through the alpaca diencephalon suggests that the peptide can be involved in many physiological actions.

AB - Introduction. The distribution of the immunoreactive cell bodies and fibers containing neurotensin in the alpaca diencephalon was determined by an immunohistochemical technique. Material and methods. The study was carried out in four male alpacas that lived at sea level. Brains of deeply anesthetized animals were fixed by perfusion with 4% paraformaldehyde. Cryostat sections were stained by a standard immunohistochemical method.Results. Cell bodies containing neurotensin were observed in the zona incerta and hypothalamus. A low/moderate density of these cell bodies was observed in the lateral hypothalamic area, anterior and dorsal hypothalamic areas, suprachiasmatic nucleus, periventricular region of the hypothalamus and in the ventromedial hypothalamic nucleus. In both thalamus and hypothalamus, immunoreactive fibers showed a widespread distribution. In the thalamus, a high density of these fibers was mainly found in the midline nuclei, whereas in the hypothalamus a high density was in general observed in the whole structure.Conclusions. In comparison with other mammals, the thalamus of the alpaca showed the most widespread distribution of neurotensin-immunoreactive fibers. The widespread distribution of neurotensin through the alpaca diencephalon suggests that the peptide can be involved in many physiological actions.

KW - Camelid

KW - Hypothalamus

KW - IHC

KW - Lama pacos

KW - Thalamus

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U2 - 10.5603/FHC.a2018.0003

DO - 10.5603/FHC.a2018.0003

M3 - Artículo

VL - 56

SP - 49

EP - 58

JO - Folia Histochemica et Cytobiologica

JF - Folia Histochemica et Cytobiologica

SN - 0239-8508

IS - 1

ER -