Study of the allelic variants CYP2C9*2 and CYP2C9*3 in the Peruvian mestizo population

Ángel Tito Alvarado, Ana María Muñoz, Berta Loja, Jessica Michiko Miyasato, Jorge Antonio García, Roberto Andrés Cerro, Luis Abel Quiñones, Nelson Miguel Varela

Resultado de la investigación: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

Resumen

Instituto Nacional de Salud. Introduction: CYP2C9 metabolizes approximately 15% of the prescribed drugs. Its gene has alleles whose frequencies differ between ethnic groups and populations. The alleles CYP2C9*2 and CYP2C9*3, account for an enzyme with decreased activity, and their frequencies have not been determined in the Peruvian mestizo population. Objective: To characterize the frequencies of the allelic variants *2 (rs1799853) and *3 (rs1057910) of CYP2C9 gen, in the Peruvian mestizo population from Lima, Tacna y Junín. Materials and methods: An observational, prospective cross-sectional with non-probabilistic sampling, for convenience and incidental was performed. 218 subjects after signing the informed consent, were incorporated according to the inclusion and exclusion criteria. Genomic DNA buccal tissue sample was obtained by swab. The detection of the genotypes CYP2C9*2 and CYP2C9*3 was made by the real-time polymerase chain reaction system using TaqMan probes. Results: Genotyping results revealed that CYP2C9*2 and CYP2C9*3 variants have low frequencies (0.046 and 0.062, respectively). The frequency of intermediate metabolizers was 15.13% (CYP2C9*1/*2: 5.96%; CYP2C9*1/*3: 9.17%) and of slow metabolizers it was 3,22% (CYP2C9*2/*2: 1.38%; CYP2C9*3/*3: 1.38%; CYP2C9*2/*3: 0.46%). Conclusions: It was possible to determine the genotypic and allelic frequencies for the variants *2 and *3 of the CYP2C9 gene in a non-probabilistic sample of the Peruvian mestizo population. The frequencies obtained (0.046 and 0.062, respectively) are among those expected for a South American mestizo population with Amerindian, European, African and Asian descent
Idioma originalEspañol
Páginas (desde-hasta)1-27
Número de páginas27
PublicaciónBiomedica
Volumen39
N.º3
DOI
EstadoPublicada - 1 ene 2019

Huella dactilar

Population
ethnic group
exclusion
inclusion
Cytochrome P-450 CYP2C9
drug
Genes
Cheek
Polymerase chain reaction
Informed Consent
Ethnic Groups
Gene Frequency
Real-Time Polymerase Chain Reaction
Alleles
Genotype
Tissue
Sampling
DNA
Enzymes
Pharmaceutical Preparations

Palabras clave

  • Cytochrome P-450 CYP2C9
  • Genotype
  • Peru
  • Pharmacogenetics

Citar esto

Alvarado, Ángel Tito ; Muñoz, Ana María ; Loja, Berta ; Miyasato, Jessica Michiko ; García, Jorge Antonio ; Cerro, Roberto Andrés ; Quiñones, Luis Abel ; Varela, Nelson Miguel. / Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en población mestiza peruana. En: Biomedica. 2019 ; Vol. 39, N.º 3. pp. 1-27.
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title = "Estudio de las variantes al{\'e}licas CYP2C9*2 y CYP2C9*3 en poblaci{\'o}n mestiza peruana",
abstract = "Instituto Nacional de Salud. Introduction: CYP2C9 metabolizes approximately 15{\%} of the prescribed drugs. Its gene has alleles whose frequencies differ between ethnic groups and populations. The alleles CYP2C9*2 and CYP2C9*3, account for an enzyme with decreased activity, and their frequencies have not been determined in the Peruvian mestizo population. Objective: To characterize the frequencies of the allelic variants *2 (rs1799853) and *3 (rs1057910) of CYP2C9 gen, in the Peruvian mestizo population from Lima, Tacna y Jun{\'i}n. Materials and methods: An observational, prospective cross-sectional with non-probabilistic sampling, for convenience and incidental was performed. 218 subjects after signing the informed consent, were incorporated according to the inclusion and exclusion criteria. Genomic DNA buccal tissue sample was obtained by swab. The detection of the genotypes CYP2C9*2 and CYP2C9*3 was made by the real-time polymerase chain reaction system using TaqMan probes. Results: Genotyping results revealed that CYP2C9*2 and CYP2C9*3 variants have low frequencies (0.046 and 0.062, respectively). The frequency of intermediate metabolizers was 15.13{\%} (CYP2C9*1/*2: 5.96{\%}; CYP2C9*1/*3: 9.17{\%}) and of slow metabolizers it was 3,22{\%} (CYP2C9*2/*2: 1.38{\%}; CYP2C9*3/*3: 1.38{\%}; CYP2C9*2/*3: 0.46{\%}). Conclusions: It was possible to determine the genotypic and allelic frequencies for the variants *2 and *3 of the CYP2C9 gene in a non-probabilistic sample of the Peruvian mestizo population. The frequencies obtained (0.046 and 0.062, respectively) are among those expected for a South American mestizo population with Amerindian, European, African and Asian descent",
keywords = "Cytochrome P-450 CYP2C9, Genotype, Peru, Pharmacogenetics",
author = "Alvarado, {{\'A}ngel Tito} and Mu{\~n}oz, {Ana Mar{\'i}a} and Berta Loja and Miyasato, {Jessica Michiko} and Garc{\'i}a, {Jorge Antonio} and Cerro, {Roberto Andr{\'e}s} and Qui{\~n}ones, {Luis Abel} and Varela, {Nelson Miguel}",
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journal = "Biomedica",
issn = "0120-4157",
publisher = "Instituto Nacional de Salud",
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Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en población mestiza peruana. / Alvarado, Ángel Tito; Muñoz, Ana María; Loja, Berta; Miyasato, Jessica Michiko; García, Jorge Antonio; Cerro, Roberto Andrés; Quiñones, Luis Abel; Varela, Nelson Miguel.

En: Biomedica, Vol. 39, N.º 3, 01.01.2019, p. 1-27.

Resultado de la investigación: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

TY - JOUR

T1 - Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en población mestiza peruana

AU - Alvarado, Ángel Tito

AU - Muñoz, Ana María

AU - Loja, Berta

AU - Miyasato, Jessica Michiko

AU - García, Jorge Antonio

AU - Cerro, Roberto Andrés

AU - Quiñones, Luis Abel

AU - Varela, Nelson Miguel

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Instituto Nacional de Salud. Introduction: CYP2C9 metabolizes approximately 15% of the prescribed drugs. Its gene has alleles whose frequencies differ between ethnic groups and populations. The alleles CYP2C9*2 and CYP2C9*3, account for an enzyme with decreased activity, and their frequencies have not been determined in the Peruvian mestizo population. Objective: To characterize the frequencies of the allelic variants *2 (rs1799853) and *3 (rs1057910) of CYP2C9 gen, in the Peruvian mestizo population from Lima, Tacna y Junín. Materials and methods: An observational, prospective cross-sectional with non-probabilistic sampling, for convenience and incidental was performed. 218 subjects after signing the informed consent, were incorporated according to the inclusion and exclusion criteria. Genomic DNA buccal tissue sample was obtained by swab. The detection of the genotypes CYP2C9*2 and CYP2C9*3 was made by the real-time polymerase chain reaction system using TaqMan probes. Results: Genotyping results revealed that CYP2C9*2 and CYP2C9*3 variants have low frequencies (0.046 and 0.062, respectively). The frequency of intermediate metabolizers was 15.13% (CYP2C9*1/*2: 5.96%; CYP2C9*1/*3: 9.17%) and of slow metabolizers it was 3,22% (CYP2C9*2/*2: 1.38%; CYP2C9*3/*3: 1.38%; CYP2C9*2/*3: 0.46%). Conclusions: It was possible to determine the genotypic and allelic frequencies for the variants *2 and *3 of the CYP2C9 gene in a non-probabilistic sample of the Peruvian mestizo population. The frequencies obtained (0.046 and 0.062, respectively) are among those expected for a South American mestizo population with Amerindian, European, African and Asian descent

AB - Instituto Nacional de Salud. Introduction: CYP2C9 metabolizes approximately 15% of the prescribed drugs. Its gene has alleles whose frequencies differ between ethnic groups and populations. The alleles CYP2C9*2 and CYP2C9*3, account for an enzyme with decreased activity, and their frequencies have not been determined in the Peruvian mestizo population. Objective: To characterize the frequencies of the allelic variants *2 (rs1799853) and *3 (rs1057910) of CYP2C9 gen, in the Peruvian mestizo population from Lima, Tacna y Junín. Materials and methods: An observational, prospective cross-sectional with non-probabilistic sampling, for convenience and incidental was performed. 218 subjects after signing the informed consent, were incorporated according to the inclusion and exclusion criteria. Genomic DNA buccal tissue sample was obtained by swab. The detection of the genotypes CYP2C9*2 and CYP2C9*3 was made by the real-time polymerase chain reaction system using TaqMan probes. Results: Genotyping results revealed that CYP2C9*2 and CYP2C9*3 variants have low frequencies (0.046 and 0.062, respectively). The frequency of intermediate metabolizers was 15.13% (CYP2C9*1/*2: 5.96%; CYP2C9*1/*3: 9.17%) and of slow metabolizers it was 3,22% (CYP2C9*2/*2: 1.38%; CYP2C9*3/*3: 1.38%; CYP2C9*2/*3: 0.46%). Conclusions: It was possible to determine the genotypic and allelic frequencies for the variants *2 and *3 of the CYP2C9 gene in a non-probabilistic sample of the Peruvian mestizo population. The frequencies obtained (0.046 and 0.062, respectively) are among those expected for a South American mestizo population with Amerindian, European, African and Asian descent

KW - Cytochrome P-450 CYP2C9

KW - Genotype

KW - Peru

KW - Pharmacogenetics

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U2 - 10.7705/biomedica.v39i4.4636

DO - 10.7705/biomedica.v39i4.4636

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JO - Biomedica

JF - Biomedica

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