Development and validation of loop-mediated isothermal amplification for the detection of the zika virus

Oscar Roberto Escalante-Maldonado, Ronnie Gustavo Gavilán, Maria Paquita García, Adolfo Marcelo, Edson Pacheco, César Cabezas, Wataru Yamazaki

Resultado de la investigación: Contribución a una revistaArtículo

Resumen

© 2019, Instituto Nacional de Salud. All rights reserved. A Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) method was developed to detect Zika. The primers were designed based on the NS5 region of 64 complete genomes. Lyophilized LAMP reagent was used. Initially, seven different arboviruses were tested and only Zika samples tested positive. Additionally, serial dilutions of one of Zika's RNA were compared using RT-LAMP and qRT-PCR, demonstrating that RT-LAMP is 1,000 times more sensitive. We also evaluated 300 serum samples with RT-LAMP comparing the results with standard qRT-PCR methods, and we obtained a 99.3% sensitivity, 100% specificity, 100% positive predictive value, and 99.3% negative predictive value. In conclusion, this method provides a low-cost, high-performance, viable, and reliable alternative for the rapid diagnosis of Zika in primary health-care facilities.
Idioma originalInglés estadounidense
Páginas (desde-hasta)442-447
Número de páginas6
PublicaciónRevista Peruana de Medicina Experimental y Salud Publica
DOI
EstadoPublicada - 1 ene 2019
Publicado de forma externa

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