Cytoprotective Effect of the Eryngium foetidum “Sacha Culantro” Methanolic Leaf Extract versus Sodium Fluoride Exposed Mice using the Micronucleus Test and the Comet Assay

Paola Raunelli, Danae Liviac, Rafael Alvis, Silvio Puente, Ivan Best, Oscar Reátegui

Resultado de la investigación: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

Resumen

Introduction: Eryngium foetidum, commonly known as sacha culantro, is a plant that grows in the Peruvian Amazonian jungle, studied for its high concentration of antioxidant substances and various ethnopharmacological properties. The main goal of the study is to observe the cytoprotective and antioxidant effect of the extract from its leafs in Mus musculus previously administered with Sodium fluoride (NaF) using the Micronucleus test and the Comet assay. Methods: The extract was administrated orally in four different concentrations: 180, 360, 720, 1440 mg/Kg for ten days, at the 11th day a single dose of NaF was administrated via intraperitoneal at 20 mg/Kg. The genotoxicity study was made with mice from the strain BALB/c, using the Micronucleus test on bone marrow and the Comet assay on peripheral blood according the OECD guides 474 and 489 respectively. The statistical analysis was made by the media’s analysis with ANOVA. Results: Significant differences were found in Micronucleus frequency between the highest concentrations of Eryngium foetidum and NaF. The Comet assay showed significant reduction in NaF induced damage according to different concentrations of the extract. Conclusion: The Eryngium foetidum extract has cytoprotective activity against oxidant substances such as NaF.

Idioma originalInglés
Páginas (desde-hasta)461-465
Número de páginas5
PublicaciónPharmacognosy Journal
Volumen11
N.º3
DOI
EstadoPublicada - 1 ene 2019

Huella dactilar

Eryngium
Sodium Fluoride
Micronucleus Tests
Comet Assay
Antioxidants
Oxidants
Analysis of Variance
Bone Marrow

Citar esto

@article{4517b7b662744684a88ee782c8f16980,
title = "Cytoprotective Effect of the Eryngium foetidum “Sacha Culantro” Methanolic Leaf Extract versus Sodium Fluoride Exposed Mice using the Micronucleus Test and the Comet Assay",
abstract = "Introduction: Eryngium foetidum, commonly known as sacha culantro, is a plant that grows in the Peruvian Amazonian jungle, studied for its high concentration of antioxidant substances and various ethnopharmacological properties. The main goal of the study is to observe the cytoprotective and antioxidant effect of the extract from its leafs in Mus musculus previously administered with Sodium fluoride (NaF) using the Micronucleus test and the Comet assay. Methods: The extract was administrated orally in four different concentrations: 180, 360, 720, 1440 mg/Kg for ten days, at the 11th day a single dose of NaF was administrated via intraperitoneal at 20 mg/Kg. The genotoxicity study was made with mice from the strain BALB/c, using the Micronucleus test on bone marrow and the Comet assay on peripheral blood according the OECD guides 474 and 489 respectively. The statistical analysis was made by the media’s analysis with ANOVA. Results: Significant differences were found in Micronucleus frequency between the highest concentrations of Eryngium foetidum and NaF. The Comet assay showed significant reduction in NaF induced damage according to different concentrations of the extract. Conclusion: The Eryngium foetidum extract has cytoprotective activity against oxidant substances such as NaF.",
keywords = "Comet assay, Cytoprotective, Eryngium foetidum, Genotoxicity, Micronucleus test, Toxicity",
author = "Paola Raunelli and Danae Liviac and Rafael Alvis and Silvio Puente and Ivan Best and Oscar Re{\'a}tegui",
year = "2019",
month = "1",
day = "1",
doi = "10.5530/pj.2019.11.72",
language = "Ingl{\'e}s",
volume = "11",
pages = "461--465",
journal = "Pharmacognosy Journal",
issn = "0975-3575",
publisher = "Emanuscript Services",
number = "3",

}

Cytoprotective Effect of the Eryngium foetidum “Sacha Culantro” Methanolic Leaf Extract versus Sodium Fluoride Exposed Mice using the Micronucleus Test and the Comet Assay. / Raunelli, Paola; Liviac, Danae; Alvis, Rafael; Puente, Silvio; Best, Ivan; Reátegui, Oscar.

En: Pharmacognosy Journal, Vol. 11, N.º 3, 01.01.2019, p. 461-465.

Resultado de la investigación: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

TY - JOUR

T1 - Cytoprotective Effect of the Eryngium foetidum “Sacha Culantro” Methanolic Leaf Extract versus Sodium Fluoride Exposed Mice using the Micronucleus Test and the Comet Assay

AU - Raunelli, Paola

AU - Liviac, Danae

AU - Alvis, Rafael

AU - Puente, Silvio

AU - Best, Ivan

AU - Reátegui, Oscar

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Introduction: Eryngium foetidum, commonly known as sacha culantro, is a plant that grows in the Peruvian Amazonian jungle, studied for its high concentration of antioxidant substances and various ethnopharmacological properties. The main goal of the study is to observe the cytoprotective and antioxidant effect of the extract from its leafs in Mus musculus previously administered with Sodium fluoride (NaF) using the Micronucleus test and the Comet assay. Methods: The extract was administrated orally in four different concentrations: 180, 360, 720, 1440 mg/Kg for ten days, at the 11th day a single dose of NaF was administrated via intraperitoneal at 20 mg/Kg. The genotoxicity study was made with mice from the strain BALB/c, using the Micronucleus test on bone marrow and the Comet assay on peripheral blood according the OECD guides 474 and 489 respectively. The statistical analysis was made by the media’s analysis with ANOVA. Results: Significant differences were found in Micronucleus frequency between the highest concentrations of Eryngium foetidum and NaF. The Comet assay showed significant reduction in NaF induced damage according to different concentrations of the extract. Conclusion: The Eryngium foetidum extract has cytoprotective activity against oxidant substances such as NaF.

AB - Introduction: Eryngium foetidum, commonly known as sacha culantro, is a plant that grows in the Peruvian Amazonian jungle, studied for its high concentration of antioxidant substances and various ethnopharmacological properties. The main goal of the study is to observe the cytoprotective and antioxidant effect of the extract from its leafs in Mus musculus previously administered with Sodium fluoride (NaF) using the Micronucleus test and the Comet assay. Methods: The extract was administrated orally in four different concentrations: 180, 360, 720, 1440 mg/Kg for ten days, at the 11th day a single dose of NaF was administrated via intraperitoneal at 20 mg/Kg. The genotoxicity study was made with mice from the strain BALB/c, using the Micronucleus test on bone marrow and the Comet assay on peripheral blood according the OECD guides 474 and 489 respectively. The statistical analysis was made by the media’s analysis with ANOVA. Results: Significant differences were found in Micronucleus frequency between the highest concentrations of Eryngium foetidum and NaF. The Comet assay showed significant reduction in NaF induced damage according to different concentrations of the extract. Conclusion: The Eryngium foetidum extract has cytoprotective activity against oxidant substances such as NaF.

KW - Comet assay

KW - Cytoprotective

KW - Eryngium foetidum

KW - Genotoxicity

KW - Micronucleus test

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=85068696501&partnerID=8YFLogxK

U2 - 10.5530/pj.2019.11.72

DO - 10.5530/pj.2019.11.72

M3 - Artículo

VL - 11

SP - 461

EP - 465

JO - Pharmacognosy Journal

JF - Pharmacognosy Journal

SN - 0975-3575

IS - 3

ER -