TY - JOUR
T1 - Attenuating effect of peruvian cocoa populations on the acute asthmatic response in brown norway rats
AU - Périz, Marta
AU - Pérez-Cano, Francisco J.
AU - Cambras, Trinitat
AU - Franch, Àngels
AU - Best, Ivan
AU - Pastor-Soplin, Santiago
AU - Castell, Margarida
AU - Massot-Cladera, Malén
N1 - Funding Information:
Funding: This research was funded by the National Fund for Scientific, Technology and Technological Innovation Development (FONDECYT) of the National Council of Science, Technology and Technological Innovation (CONCYTEC), Contract 137-2017-FONDECYT, and Universidad Científica del Sur. Open access fees were supported by the Universidad Cientifica del Sur (Lima, Peru).
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/7/31
Y1 - 2020/7/31
N2 - Cocoa contains bioactive components, which vary according to genetic and environmental factors. The present study aimed to ascertain the anti-allergic properties of native Peruvian cocoa populations (“Blanco de Piura” or BPC, “Amazonas Peru” or APC, “Criollo de Montaña” or CMC, “Chuncho” or CCC, and an ordinary cocoa or OC). To do so, after an initial in vitro approach, an in vivo study focused on the induction of an anaphylactic response associated with allergic asthma in Brown Norway rats was carried out. Based on their polyphenol content, antioxidant activity and in vitro effects, the APC and CMC were selected to be included in the in vivo study. Cocoa diets were tested in a model of allergic asthma in which anaphylactic response was assessed by changes in body temperature, motor activity and body weight. The concentration of specific immunoglobulin E (IgE), mast cell protease and leukotrienes was also quantified in serum and/or bronchoalveolar lavage fluid. CMC and OC populations exhibited a protective effect on the allergic asthma rat model as evidenced by means of a partial protection against anaphylactic response and, above all, in the synthesis of IgE and the release of mast cell protease.
AB - Cocoa contains bioactive components, which vary according to genetic and environmental factors. The present study aimed to ascertain the anti-allergic properties of native Peruvian cocoa populations (“Blanco de Piura” or BPC, “Amazonas Peru” or APC, “Criollo de Montaña” or CMC, “Chuncho” or CCC, and an ordinary cocoa or OC). To do so, after an initial in vitro approach, an in vivo study focused on the induction of an anaphylactic response associated with allergic asthma in Brown Norway rats was carried out. Based on their polyphenol content, antioxidant activity and in vitro effects, the APC and CMC were selected to be included in the in vivo study. Cocoa diets were tested in a model of allergic asthma in which anaphylactic response was assessed by changes in body temperature, motor activity and body weight. The concentration of specific immunoglobulin E (IgE), mast cell protease and leukotrienes was also quantified in serum and/or bronchoalveolar lavage fluid. CMC and OC populations exhibited a protective effect on the allergic asthma rat model as evidenced by means of a partial protection against anaphylactic response and, above all, in the synthesis of IgE and the release of mast cell protease.
KW - Anaphylaxis
KW - Asthma
KW - IgE
KW - Leukotriene
KW - Mast cell protease
KW - Methylxanthines
KW - Motor activity
KW - Polyphenols
KW - Temperature
KW - Theobroma cacao
UR - http://www.scopus.com/inward/record.url?scp=85089008344&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/8dc80072-14e4-312d-84a5-43a6914c02e2/
U2 - 10.3390/nu12082301
DO - 10.3390/nu12082301
M3 - Artículo
C2 - 32751867
AN - SCOPUS:85089008344
SN - 2072-6643
VL - 12
SP - 1
EP - 21
JO - Nutrients
JF - Nutrients
IS - 8
M1 - 2301
ER -