Antimicrobial-producing Pseudoalteromonas from the marine environment of Panama shows a high phylogenetic diversity and clonal structure

Librada A. Atencio, Francesco Dal Grande, Giannina Ow Young, Ronnie Gavilán, Héctor M. Guzmán, Imke Schmitt, Luis C. Mejía, Marcelino Gutiérrez

Resultado de la investigación: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

Resumen

© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Pseudoalteromonas is a genus of marine bacteria often found in association with other organisms. Although several studies have examined Pseudoalteromonas diversity and their antimicrobial activity, its diversity in tropical environments is largely unexplored. We investigated the diversity of Pseudoalteromonas in marine environments of Panama using a multilocus phylogenetic approach. Furthermore we tested their antimicrobial capacity and evaluated the effect of recombination and mutation in shaping their phylogenetic relationships. The reconstruction of clonal relationships among 78 strains including 15 reference Pseudoalteromonas species revealed 43 clonal lineages, divided in pigmented and non-pigmented strains. In total, 39 strains displayed moderate to high activity against Gram-positive and Gram-negative bacteria and fungi. Linkage disequilibrium analyses showed that the Pseudoalteromonas strains of Panama have a highly clonal structure and that, although present, recombination is not frequent enough to break the association among alleles. This clonal structure is in contrast to the high rates of recombination generally reported for aquatic and marine bacteria. We propose that this structure is likely due to the symbiotic association with marine invertebrates of most strains analyzed. Our results also show that there are several putative new species of Pseudoalteromonas in Panama to be described.
Idioma originalInglés estadounidense
Páginas (desde-hasta)747-769
Número de páginas23
PublicaciónJournal of Basic Microbiology
DOI
EstadoPublicada - 1 sep 2018

Huella dactilar

Pseudoalteromonas
Panama
Genetic Recombination
Bacteria
Linkage Disequilibrium
Invertebrates
Gram-Negative Bacteria
Fungi
Alleles
Mutation

Citar esto

Atencio, Librada A. ; Dal Grande, Francesco ; Young, Giannina Ow ; Gavilán, Ronnie ; Guzmán, Héctor M. ; Schmitt, Imke ; Mejía, Luis C. ; Gutiérrez, Marcelino. / Antimicrobial-producing Pseudoalteromonas from the marine environment of Panama shows a high phylogenetic diversity and clonal structure. En: Journal of Basic Microbiology. 2018 ; pp. 747-769.
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abstract = "{\circledC} 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Pseudoalteromonas is a genus of marine bacteria often found in association with other organisms. Although several studies have examined Pseudoalteromonas diversity and their antimicrobial activity, its diversity in tropical environments is largely unexplored. We investigated the diversity of Pseudoalteromonas in marine environments of Panama using a multilocus phylogenetic approach. Furthermore we tested their antimicrobial capacity and evaluated the effect of recombination and mutation in shaping their phylogenetic relationships. The reconstruction of clonal relationships among 78 strains including 15 reference Pseudoalteromonas species revealed 43 clonal lineages, divided in pigmented and non-pigmented strains. In total, 39 strains displayed moderate to high activity against Gram-positive and Gram-negative bacteria and fungi. Linkage disequilibrium analyses showed that the Pseudoalteromonas strains of Panama have a highly clonal structure and that, although present, recombination is not frequent enough to break the association among alleles. This clonal structure is in contrast to the high rates of recombination generally reported for aquatic and marine bacteria. We propose that this structure is likely due to the symbiotic association with marine invertebrates of most strains analyzed. Our results also show that there are several putative new species of Pseudoalteromonas in Panama to be described.",
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Antimicrobial-producing Pseudoalteromonas from the marine environment of Panama shows a high phylogenetic diversity and clonal structure. / Atencio, Librada A.; Dal Grande, Francesco; Young, Giannina Ow; Gavilán, Ronnie; Guzmán, Héctor M.; Schmitt, Imke; Mejía, Luis C.; Gutiérrez, Marcelino.

En: Journal of Basic Microbiology, 01.09.2018, p. 747-769.

Resultado de la investigación: Contribución a una revistaArtículoInvestigaciónrevisión exhaustiva

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T1 - Antimicrobial-producing Pseudoalteromonas from the marine environment of Panama shows a high phylogenetic diversity and clonal structure

AU - Atencio, Librada A.

AU - Dal Grande, Francesco

AU - Young, Giannina Ow

AU - Gavilán, Ronnie

AU - Guzmán, Héctor M.

AU - Schmitt, Imke

AU - Mejía, Luis C.

AU - Gutiérrez, Marcelino

PY - 2018/9/1

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N2 - © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Pseudoalteromonas is a genus of marine bacteria often found in association with other organisms. Although several studies have examined Pseudoalteromonas diversity and their antimicrobial activity, its diversity in tropical environments is largely unexplored. We investigated the diversity of Pseudoalteromonas in marine environments of Panama using a multilocus phylogenetic approach. Furthermore we tested their antimicrobial capacity and evaluated the effect of recombination and mutation in shaping their phylogenetic relationships. The reconstruction of clonal relationships among 78 strains including 15 reference Pseudoalteromonas species revealed 43 clonal lineages, divided in pigmented and non-pigmented strains. In total, 39 strains displayed moderate to high activity against Gram-positive and Gram-negative bacteria and fungi. Linkage disequilibrium analyses showed that the Pseudoalteromonas strains of Panama have a highly clonal structure and that, although present, recombination is not frequent enough to break the association among alleles. This clonal structure is in contrast to the high rates of recombination generally reported for aquatic and marine bacteria. We propose that this structure is likely due to the symbiotic association with marine invertebrates of most strains analyzed. Our results also show that there are several putative new species of Pseudoalteromonas in Panama to be described.

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