Monkeypox is causing great concern in society because of its great infective power and the possibility that it could become a new pandemic. This study aimed to adapt and validate the monkeypox concern scale in the Peruvian adult population (EP-VIR-MONK). An instrumental cross-sectional study was carried out under a non-probabilistic convenience sampling with 779 adults from the three regions of Peru (coast, highlands, and jungle). The instrument was adapted based on an instrument previously validated related to the concern caused by COVID-19 in Peru. Content-based validity was calculated with Aiken’s V coefficient, internal structure with confirmatory factor analysis, and reliability with the omega coefficient. The first evidence of validity of EP-VIR-MONK based on its content, internal structure, and reliability in a sample of Peruvian adults is presented here. Accordingly, it can be helpful for the management of the prevention of mental health alterations resulting from the proliferation of monkeypox in Peru.