Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: A systematic review

Aaron Lloyd, Vinay Pasupuleti, Priyaleela Thota, Chaitanya Pant, David D.K. Rolston, Adrian V. Hernández, Vicente Benites Zapata, Thomas G. Fraser, Curtis J. Donskey, Abhishek Deshpande

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)

Resumen

Loop-mediated isothermal DNA amplification (LAMP) is currently used as standalone diagnostic test for C. difficile infection (CDI). We assessed the diagnostic accuracy of LAMP for the diagnosis of CDI. We searched 5 databases to identify studies that compared LAMP with culture cytotoxicity neutralization assay or anaerobic toxigenic culture (TC) of C. difficile. We used the random-effects model to calculate pooled sensitivities, specificities, diagnostic odds ratios, and their 95% confidence intervals (CIs). The search of the databases yielded 16 studies (6979 samples) that met inclusion criteria. When TC was used as the gold standard (6572 samples), bivariate analysis yielded a mean sensitivity of 0.95 (95% CI, 0.93-0.97; I2=67.4) and a mean specificity of 0.99 (95% CI, 0.96-1.00; I2=97.0). LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting CDI. The results should, however, be interpreted only in the presence of clinical suspicion and symptoms of CDI.

Idioma originalInglés
Páginas (desde-hasta)4-10
Número de páginas7
PublicaciónDiagnostic Microbiology and Infectious Disease
Volumen82
N.º1
DOI
EstadoPublicada - 1 may 2015
Publicado de forma externa

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