Frecuencia de resistencia a la colistina en pseudomonas aeruginosa: Primer reporte en el Perú

Translated title of the contribution: Frequency of colistin resistance in pseudomonas aeruginosa: First report from Peru

Marjhory Zarate, Delsy Barrantes, Diego Cuicapuza, Jorge Velasquez, Nathaly Fernández, Guillermo Salvatierra, Jesus Tamariz

Research output: Contribution to journalArticlepeer-review

Abstract

This study aimed to determine the frequency of colistin resistance in Pseudomonas aeruginosa isolates obtained from three healthcare facilities in Lima and cryopreserved at the Laboratorio de Resistencia Antimicrobianos e Inmunopatología of the Universidad Peruana Cayetano Heredia (UPCH). The co-listin broth disk elution method was used for the phenotypic identification of colistin resistance. We detected the expression of the mcr-1 gene by using the phenotypic diffusion method with combined colistin and ethylenediaminetetraacetic acid (EDTA) disks; and polymerase chain reaction (PCR) was used for molecular identification of the gene. Of the 97 isolates, 7 (7.2%) were resistant to colistin; howe-ver, none carried the mcr-1 gene. This is the first report from Peru on clinical isolates of colistin-resistant Pseudomonas aeruginosa, which suggests the need for implementation of appropriate methodologies for the epidemiological surveillance of colistin-resistant pathogens.

Translated title of the contributionFrequency of colistin resistance in pseudomonas aeruginosa: First report from Peru
Original languageSpanish
Pages (from-to)308-312
Number of pages5
JournalRevista Peruana de Medicina Experimental y Salud Publica
Volume38
Issue number2
DOIs
StatePublished - 26 May 2021
Externally publishedYes

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