Evaluation of reverse transcription-loop-mediated isothermal amplification for rapid detection of SARS-CoV-2

Willi Quino; Diana Flores-León; Junior Caro-Castro; Carmen V. Hurtado; Iris Silva; Ronnie G. Gavilan

doi:10.1038/s41598-021-03623-y

Evaluation of reverse transcription-loop-mediated isothermal amplification for rapid detection of SARS-CoV-2

Willi Quino, Diana Flores-León, Junior Caro-Castro, Carmen V. Hurtado, Iris Silva, Ronnie G. Gavilan

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The main strategy for response and control of COVID-19 demands the use of rapid, accurate diagnostic tests aimed at the first point of health care. During the emergency, an increase in asymptomatic and symptomatic cases results in a great demand for molecular tests, which is promoting the development and application of rapid diagnostic technologies. In this study, we describe the development and evaluation of RT-LAMP to detect SARS-CoV-2 based on three genes (ORF1ab, M and N genes) in monoplex and triplex format. RT-LAMP assays were compared with the gold standard method RT-qPCR. The triplex format (RdRp, M and N genes) allowed obtaining comparable results with de RT-qPCR (RdRp and E genes), presented a sensitivity of 98.9% and a specificity of 97.9%, opening the opportunity to apply this method to detect SARS-CoV-2 at primary health-care centers.

Original language	English
Article number	24234
Journal	Scientific Reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-03623-y
State	Published - Dec 2021
Externally published	Yes

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abstract = "The main strategy for response and control of COVID-19 demands the use of rapid, accurate diagnostic tests aimed at the first point of health care. During the emergency, an increase in asymptomatic and symptomatic cases results in a great demand for molecular tests, which is promoting the development and application of rapid diagnostic technologies. In this study, we describe the development and evaluation of RT-LAMP to detect SARS-CoV-2 based on three genes (ORF1ab, M and N genes) in monoplex and triplex format. RT-LAMP assays were compared with the gold standard method RT-qPCR. The triplex format (RdRp, M and N genes) allowed obtaining comparable results with de RT-qPCR (RdRp and E genes), presented a sensitivity of 98.9% and a specificity of 97.9%, opening the opportunity to apply this method to detect SARS-CoV-2 at primary health-care centers.",

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AU - Silva, Iris

AU - Gavilan, Ronnie G.

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Evaluation of reverse transcription-loop-mediated isothermal amplification for rapid detection of SARS-CoV-2

Abstract

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