Effect of a natural supplement containing glucosinolates, phytosterols and citrus flavonoids on body weight and metabolic parameters in a menopausal murine model induced by bilateral ovariectomy

Martha Valdivia, Percy Soto-Becerra, Ricardo Laguna-Barraza, Percy A. Rojas, Ivonne Reyes-Mandujano, Paola Gonzáles-Reyes, Haydee Temoche, Olga S. Timoteo, Gabriela Lugo-Martinez, Claudia C. Calzada-Mendoza, Edward Mezones Holguin

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To evaluate the effect of a herbal preparation containing glucosinolates, phytosterols and citrus flavonoids (supplement) on body weight and metabolic parameters usually impaired by menopause. Methods: A pre-clinical experimental study carried out in twenty-five Swiss strain mice (Mus musculus) randomly distributed (1:1:1:1:1 ratio) to five groups to receive for ten weeks: (1) oral gelatinized maca extract 0.5625 mg/kg/day + bilateral ovariectomy (Maca + OVX); (2) oral supplement 0.5625 mg/kg/day + bilateral ovariectomy (S1 + OVX); (3) oral supplement 1.6875 mg/kg/day + bilateral ovariectomy (S2 + OVX); (4) oral saline 100 µl/kg/day + bilateral ovariectomy (OVX); and (5) oral saline 100 µl/kg/day + sham surgery (sham). The primary endpoint was change in body weight gain from baseline to final. Secondary endpoints were uterine weight and cholesterol, triglyceride, glucose, and glucose/triglycerides index values at the end of the study. A modified intention-to-treat analysis was performed through linear regression models and using the Bonferroni method to penalized p-values by multiple comparisons. Results: Twenty-three animals completed the study. There was a significant average difference in weight gain, with a greater reduction in the S2 + OVX group compared to the OVX group (difference= −3.5; 95% CI (–5.27; −1.74); p <.001). S2 + OVX group also displayed a significant average reduction of total blood cholesterol (difference: −16.94; 95% CI (–33.73; −0.15); p =.037). No significant effects of the supplement were found on other secondary endpoints. Conclusion: In this murine menopausal model, triple oral supplement dose resulted in an average reduction of weight gain and total cholesterol levels, suggesting that the compound could have a potential effect at regulating menopausal altered metabolism.

Original languageEnglish
Pages (from-to)1106-1111
Number of pages6
JournalGynecological Endocrinology
Volume36
Issue number12
DOIs
StatePublished - 2020
Externally publishedYes

Keywords

  • body weight
  • citrus flavonoids
  • glucosinolates
  • Lepidium Meyenii
  • menopause
  • metabolism
  • phytosterol
  • supplementation

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