Development and validation of loop-mediated isothermal amplification for the detection of the zika virus

Oscar Roberto Escalante-Maldonado, Ronnie Gustavo Gavilán, Maria Paquita García, Adolfo Marcelo, Edson Pacheco, César Cabezas, Wataru Yamazaki

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

© 2019, Instituto Nacional de Salud. All rights reserved. A Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) method was developed to detect Zika. The primers were designed based on the NS5 region of 64 complete genomes. Lyophilized LAMP reagent was used. Initially, seven different arboviruses were tested and only Zika samples tested positive. Additionally, serial dilutions of one of Zika's RNA were compared using RT-LAMP and qRT-PCR, demonstrating that RT-LAMP is 1,000 times more sensitive. We also evaluated 300 serum samples with RT-LAMP comparing the results with standard qRT-PCR methods, and we obtained a 99.3% sensitivity, 100% specificity, 100% positive predictive value, and 99.3% negative predictive value. In conclusion, this method provides a low-cost, high-performance, viable, and reliable alternative for the rapid diagnosis of Zika in primary health-care facilities.
Original languageAmerican English
Pages (from-to)442-447
Number of pages6
JournalRevista Peruana de Medicina Experimental y Salud Publica
DOIs
StatePublished - 1 Jan 2019
Externally publishedYes

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