TY - JOUR
T1 - Development and characterization of an allergic asthma rat model for interventional studies
AU - Périz, Marta
AU - Pérez-Cano, Francisco J.
AU - Rodríguez-Lagunas, Maria J.
AU - Cambras, Trinitat
AU - Pastor-Soplin, Santiago
AU - Best, Iván
AU - Castell, Margarida
AU - Massot-Cladera, Malén
N1 - Funding Information:
Funding: This research was funded by the National Fund for Scientific, Technology and Technological Innovation Development (FONDECYT) of the National Council of Science, Technology and Technological Innovation (CONCYTEC), Contract 137-2017-FONDECYT.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/5/28
Y1 - 2020/5/28
N2 - Allergic asthma is one of the most common chronic diseases of the airways, however it still remains underdiagnosed and hence undertreated. Therefore, an allergic asthma rat model would be useful to be applied in future therapeutic strategy studies. The aim of the present study was to develop an objective model of allergic asthma in atopic rats that allows the induction and quantification of anaphylactic shock with quantitative variables. Female Brown Norway rats were intraperitoneally sensitized with ovalbumin (OVA), alum and Bordetella pertussis toxin and boosted a week later with OVA in alum. At day 28, all rats received an intranasal challenge with OVA. Anaphylactic response was accurately assessed by changes in motor activity and body temperature. Leukotriene concentration was determined in the bronchoalveolar lavage fluid (BALF), and total and IgE anti-OVA antibodies were quantified in blood and BALF samples. The asthmatic animals’ motility and body temperature were reduced after the shock for at least 20 h. The asthmatic animals developed anti-OVA IgE antibodies both in BALF and in serum. These results show an effective and relatively rapid model of allergic asthma in female Brown Norway rats that allows the quantification of the anaphylactic response.
AB - Allergic asthma is one of the most common chronic diseases of the airways, however it still remains underdiagnosed and hence undertreated. Therefore, an allergic asthma rat model would be useful to be applied in future therapeutic strategy studies. The aim of the present study was to develop an objective model of allergic asthma in atopic rats that allows the induction and quantification of anaphylactic shock with quantitative variables. Female Brown Norway rats were intraperitoneally sensitized with ovalbumin (OVA), alum and Bordetella pertussis toxin and boosted a week later with OVA in alum. At day 28, all rats received an intranasal challenge with OVA. Anaphylactic response was accurately assessed by changes in motor activity and body temperature. Leukotriene concentration was determined in the bronchoalveolar lavage fluid (BALF), and total and IgE anti-OVA antibodies were quantified in blood and BALF samples. The asthmatic animals’ motility and body temperature were reduced after the shock for at least 20 h. The asthmatic animals developed anti-OVA IgE antibodies both in BALF and in serum. These results show an effective and relatively rapid model of allergic asthma in female Brown Norway rats that allows the quantification of the anaphylactic response.
KW - Body temperature
KW - Bronchoalveolar lavage fluid
KW - Brown Norway rats
KW - IgE
KW - Intranasal challenge
KW - Leukotriene
KW - Motor activity
KW - Ovalbumin
UR - http://www.scopus.com/inward/record.url?scp=85085676194&partnerID=8YFLogxK
U2 - 10.3390/ijms21113841
DO - 10.3390/ijms21113841
M3 - Artículo
C2 - 32481675
AN - SCOPUS:85085676194
SN - 1661-6596
VL - 21
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 11
M1 - 3841
ER -
