Development and characterization of an allergic asthma rat model for interventional studies

Marta Périz, Francisco J. Pérez-Cano, Maria J. Rodríguez-Lagunas, Trinitat Cambras, Santiago Pastor-Soplin, Iván Best, Margarida Castell, Malén Massot-Cladera

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Allergic asthma is one of the most common chronic diseases of the airways, however it still remains underdiagnosed and hence undertreated. Therefore, an allergic asthma rat model would be useful to be applied in future therapeutic strategy studies. The aim of the present study was to develop an objective model of allergic asthma in atopic rats that allows the induction and quantification of anaphylactic shock with quantitative variables. Female Brown Norway rats were intraperitoneally sensitized with ovalbumin (OVA), alum and Bordetella pertussis toxin and boosted a week later with OVA in alum. At day 28, all rats received an intranasal challenge with OVA. Anaphylactic response was accurately assessed by changes in motor activity and body temperature. Leukotriene concentration was determined in the bronchoalveolar lavage fluid (BALF), and total and IgE anti-OVA antibodies were quantified in blood and BALF samples. The asthmatic animals’ motility and body temperature were reduced after the shock for at least 20 h. The asthmatic animals developed anti-OVA IgE antibodies both in BALF and in serum. These results show an effective and relatively rapid model of allergic asthma in female Brown Norway rats that allows the quantification of the anaphylactic response.

Original languageEnglish
Article number3841
JournalInternational Journal of Molecular Sciences
Volume21
Issue number11
DOIs
StatePublished - 28 May 2020

Keywords

  • Body temperature
  • Bronchoalveolar lavage fluid
  • Brown Norway rats
  • IgE
  • Intranasal challenge
  • Leukotriene
  • Motor activity
  • Ovalbumin

Fingerprint

Dive into the research topics of 'Development and characterization of an allergic asthma rat model for interventional studies'. Together they form a unique fingerprint.

Cite this