Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos

Pool Marcos-Carbajal; Guillermo Salvatierra; José Yareta; Jimena Pino; Nancy Vásquez; Pilar Diaz; Isabel Martínez; Percy Asmat; Carlos Peralta; Caridad Huamani; Alexander Briones; Manuel Ruiz; Nicomedes Laura; Álvaro Luque; Leonel Arapa; Pablo Tsukayama

doi:10.17843/rpmesp.2021.381.6182

Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos

Translated title of the contribution: Microbiological and molecular characterization of antimicrobial resistance in uropathogenic escherichia coli from peruvian public hospitals

Pool Marcos-Carbajal, Guillermo Salvatierra, José Yareta, Jimena Pino, Nancy Vásquez, Pilar Diaz, Isabel Martínez, Percy Asmat, Carlos Peralta, Caridad Huamani, Alexander Briones, Manuel Ruiz, Nicomedes Laura, Álvaro Luque, Leonel Arapa, Pablo Tsukayama

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We characterized the antimicrobial resistance of 70 Escherichia coli isolates obtained from patients with a urinary tract infection (UTI) from 8 public hospitals in Peru. Resistance profiles were identified using the automated MicroScan® system. A standard polymerase chain reaction was used for the detection of the bla_TEM, bla_CTX-M, bla_SHV and bla_PER genes. The 65.7% (46/70) of the isolates presented a multidrug-re-sistant phenotype and 55.7% (39/70) were extended-spectrum beta-lactamases producers. High levels of resistance were detected for ampicillin (77,1%), ciprofloxacin (74,3%), trimethoprim/sulfamethoxazole (62,9%), cefepime (57,1%), and cefuroxime (57,1%). The bla_TEM gene was the most frequent (31,4%), followed by bla_CTX-M (18,6%) and bla_SHV (2,9%) genes. These results show high resistance levels to antimi-crobials of clinical use in E. coli isolates from hospital UTI patients in Peru.

Translated title of the contribution	Microbiological and molecular characterization of antimicrobial resistance in uropathogenic escherichia coli from peruvian public hospitals
Original language	Spanish
Pages (from-to)	119-123
Number of pages	5
Journal	Revista Peruana de Medicina Experimental y Salud Publica
Volume	38
Issue number	1
DOIs	https://doi.org/10.17843/rpmesp.2021.381.6182
State	Published - 1 Jan 2021
Externally published	Yes

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Marcos-Carbajal, P., Salvatierra, G., Yareta, J., Pino, J., Vásquez, N., Diaz, P., Martínez, I., Asmat, P., Peralta, C., Huamani, C., Briones, A., Ruiz, M., Laura, N., Luque, Á., Arapa, L., & Tsukayama, P. (2021). Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos. Revista Peruana de Medicina Experimental y Salud Publica, 38(1), 119-123. https://doi.org/10.17843/rpmesp.2021.381.6182

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abstract = "We characterized the antimicrobial resistance of 70 Escherichia coli isolates obtained from patients with a urinary tract infection (UTI) from 8 public hospitals in Peru. Resistance profiles were identified using the automated MicroScan{\textregistered} system. A standard polymerase chain reaction was used for the detection of the blaTEM, blaCTX-M, blaSHV and blaPER genes. The 65.7% (46/70) of the isolates presented a multidrug-re-sistant phenotype and 55.7% (39/70) were extended-spectrum beta-lactamases producers. High levels of resistance were detected for ampicillin (77,1%), ciprofloxacin (74,3%), trimethoprim/sulfamethoxazole (62,9%), cefepime (57,1%), and cefuroxime (57,1%). The blaTEM gene was the most frequent (31,4%), followed by blaCTX-M (18,6%) and blaSHV (2,9%) genes. These results show high resistance levels to antimi-crobials of clinical use in E. coli isolates from hospital UTI patients in Peru.",

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author = "Pool Marcos-Carbajal and Guillermo Salvatierra and Jos{\'e} Yareta and Jimena Pino and Nancy V{\'a}squez and Pilar Diaz and Isabel Mart{\'i}nez and Percy Asmat and Carlos Peralta and Caridad Huamani and Alexander Briones and Manuel Ruiz and Nicomedes Laura and {\'A}lvaro Luque and Leonel Arapa and Pablo Tsukayama",

year = "2021",

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doi = "10.17843/rpmesp.2021.381.6182",

language = "Espa{\~n}ol",

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Marcos-Carbajal, P, Salvatierra, G, Yareta, J, Pino, J, Vásquez, N, Diaz, P, Martínez, I, Asmat, P, Peralta, C, Huamani, C, Briones, A, Ruiz, M, Laura, N, Luque, Á, Arapa, L & Tsukayama, P 2021, 'Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos', Revista Peruana de Medicina Experimental y Salud Publica, vol. 38, no. 1, pp. 119-123. https://doi.org/10.17843/rpmesp.2021.381.6182

Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos. / Marcos-Carbajal, Pool; Salvatierra, Guillermo; Yareta, José et al.

In: Revista Peruana de Medicina Experimental y Salud Publica, Vol. 38, No. 1, 01.01.2021, p. 119-123.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos

AU - Marcos-Carbajal, Pool

AU - Salvatierra, Guillermo

AU - Yareta, José

AU - Pino, Jimena

AU - Vásquez, Nancy

AU - Diaz, Pilar

AU - Martínez, Isabel

AU - Asmat, Percy

AU - Peralta, Carlos

AU - Huamani, Caridad

AU - Briones, Alexander

AU - Ruiz, Manuel

AU - Laura, Nicomedes

AU - Luque, Álvaro

AU - Arapa, Leonel

AU - Tsukayama, Pablo

PY - 2021/1/1

Y1 - 2021/1/1

N2 - We characterized the antimicrobial resistance of 70 Escherichia coli isolates obtained from patients with a urinary tract infection (UTI) from 8 public hospitals in Peru. Resistance profiles were identified using the automated MicroScan® system. A standard polymerase chain reaction was used for the detection of the blaTEM, blaCTX-M, blaSHV and blaPER genes. The 65.7% (46/70) of the isolates presented a multidrug-re-sistant phenotype and 55.7% (39/70) were extended-spectrum beta-lactamases producers. High levels of resistance were detected for ampicillin (77,1%), ciprofloxacin (74,3%), trimethoprim/sulfamethoxazole (62,9%), cefepime (57,1%), and cefuroxime (57,1%). The blaTEM gene was the most frequent (31,4%), followed by blaCTX-M (18,6%) and blaSHV (2,9%) genes. These results show high resistance levels to antimi-crobials of clinical use in E. coli isolates from hospital UTI patients in Peru.

AB - We characterized the antimicrobial resistance of 70 Escherichia coli isolates obtained from patients with a urinary tract infection (UTI) from 8 public hospitals in Peru. Resistance profiles were identified using the automated MicroScan® system. A standard polymerase chain reaction was used for the detection of the blaTEM, blaCTX-M, blaSHV and blaPER genes. The 65.7% (46/70) of the isolates presented a multidrug-re-sistant phenotype and 55.7% (39/70) were extended-spectrum beta-lactamases producers. High levels of resistance were detected for ampicillin (77,1%), ciprofloxacin (74,3%), trimethoprim/sulfamethoxazole (62,9%), cefepime (57,1%), and cefuroxime (57,1%). The blaTEM gene was the most frequent (31,4%), followed by blaCTX-M (18,6%) and blaSHV (2,9%) genes. These results show high resistance levels to antimi-crobials of clinical use in E. coli isolates from hospital UTI patients in Peru.

KW - Anti-Bacterial Agents/pharmacology

KW - Drug Resistance, Bacterial/genetics

KW - Escherichia coli Infections/drug therapy

KW - Hospitals, Public

KW - Humans

KW - Microbial Sensitivity Tests

KW - Peru

KW - Uropathogenic Escherichia coli/genetics

KW - beta-Lactamases/genetics

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DO - 10.17843/rpmesp.2021.381.6182

M3 - Artículo

C2 - 34190903

AN - SCOPUS:85111789116

SN - 1726-4634

VL - 38

SP - 119

EP - 123

JO - Revista Peruana de Medicina de Experimental y Salud Publica

JF - Revista Peruana de Medicina de Experimental y Salud Publica

IS - 1

ER -

Caracterización microbiológica y molecular de la resistencia antimicrobiana de escherichia coli uropatógenas de hospitales públicos peruanos

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