@article{8b92f1bf4a6340848d0939b9fe20c881,
title = "An accessible, efficient and global approach for the large-scale sequencing of bacterial genomes",
abstract = "We have developed an efficient and inexpensive pipeline for streamlining large-scale collection and genome sequencing of bacterial isolates. Evaluation of this method involved a worldwide research collaboration focused on the model organism Salmonella enterica, the 10KSG consortium. Following the optimization of a logistics pipeline that involved shipping isolates as thermolysates in ambient conditions, the project assembled a diverse collection of 10,419 isolates from low- and middle-income countries. The genomes were sequenced using the LITE pipeline for library construction, with a total reagent cost of less than USD$10 per genome. Our method can be applied to other large bacterial collections to underpin global collaborations.",
keywords = "Salmonella, Thermolysates, Whole-genome sequencing, iNTS",
author = "{The 10KSG consortium} and Perez-Sepulveda, {Blanca M.} and Darren Heavens and Pulford, {Caisey V.} and Predeus, {Alexander V.} and Ross Low and Hermione Webster and Dykes, {Gregory F.} and Christian Schudoma and Will Rowe and James Lipscombe and Chris Watkins and Benjamin Kumwenda and Neil Shearer and Karl Costigan and Baker, {Kate S.} and Feasey, {Nicholas A.} and Hinton, {Jay C.D.} and Neil Hall and Perez-Sepulveda, {Blanca M.} and Darren Heavens and Pulford, {Caisey V.} and Acu{\~n}a, {Mar{\'i}a Teresa} and Dragan Antic and Martin Antonio and Baker, {Kate S.} and Johan Bernal and Hilda Bola{\~n}os and Marie Chattaway and John Cheesbrough and Angeziwa Chirambo and Karl Costigan and Saffiatou Darboe and Paula D{\'i}az and Pilar Donado and Carolina Duarte and Francisco Duarte and Dean Everett and S{\'e}amus Fanning and Feasey, {Nicholas A.} and Patrick Feglo and Ferreira, {Adriano M.} and Rachel Floyd and Gavil{\'a}n, {Ronnie G.} and Gordon, {Melita A.} and Neil Hall and Hernandes, {Rodrigo T.} and Gabriela Hern{\'a}ndez-Mora and Hinton, {Jay C.D.} and Daniel Hurley and Kasumba, {Irene N.}",
note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1186/s13059-021-02536-3",
language = "Ingl{\'e}s",
volume = "22",
journal = "Genome Biology",
issn = "1474-7596",
publisher = "BioMed Central Ltd.",
number = "1",
}