An accessible, efficient and global approach for the large-scale sequencing of bacterial genomes

The 10KSG consortium

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We have developed an efficient and inexpensive pipeline for streamlining large-scale collection and genome sequencing of bacterial isolates. Evaluation of this method involved a worldwide research collaboration focused on the model organism Salmonella enterica, the 10KSG consortium. Following the optimization of a logistics pipeline that involved shipping isolates as thermolysates in ambient conditions, the project assembled a diverse collection of 10,419 isolates from low- and middle-income countries. The genomes were sequenced using the LITE pipeline for library construction, with a total reagent cost of less than USD$10 per genome. Our method can be applied to other large bacterial collections to underpin global collaborations.

Original languageEnglish
Article number349
JournalGenome Biology
Volume22
Issue number1
DOIs
StatePublished - Dec 2021

Keywords

  • Salmonella
  • Thermolysates
  • Whole-genome sequencing
  • iNTS

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