Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: A systematic review

Aaron Lloyd; Vinay Pasupuleti; Priyaleela Thota; Chaitanya Pant; David D.K. Rolston; Adrian V. Hernández; Vicente Benites Zapata; Thomas G. Fraser; Curtis J. Donskey; Abhishek Deshpande

doi:10.1016/j.diagmicrobio.2015.02.007

Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: A systematic review

Aaron Lloyd, Vinay Pasupuleti, Priyaleela Thota, Chaitanya Pant, David D.K. Rolston, Adrian V. Hernández, Vicente Benites Zapata, Thomas G. Fraser, Curtis J. Donskey, Abhishek Deshpande

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Abstract

© 2015 Elsevier Inc. Loop-mediated isothermal DNA amplification (LAMP) is currently used as standalone diagnostic test for C. difficile infection (CDI). We assessed the diagnostic accuracy of LAMP for the diagnosis of CDI. We searched 5 databases to identify studies that compared LAMP with culture cytotoxicity neutralization assay or anaerobic toxigenic culture (TC) of C. difficile. We used the random-effects model to calculate pooled sensitivities, specificities, diagnostic odds ratios, and their 95% confidence intervals (CIs). The search of the databases yielded 16 studies (6979 samples) that met inclusion criteria. When TC was used as the gold standard (6572 samples), bivariate analysis yielded a mean sensitivity of 0.95 (95% CI, 0.93-0.97; I2=67.4) and a mean specificity of 0.99 (95% CI, 0.96-1.00; I2=97.0). LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting CDI. The results should, however, be interpreted only in the presence of clinical suspicion and symptoms of CDI.

Original language	American English
Pages (from-to)	4-10
Number of pages	7
Journal	Diagnostic Microbiology and Infectious Disease
Volume	82
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2015.02.007 https://doi.org/10.1016/j.diagmicrobio.2015.02.007
State	Published - 1 May 2015
Externally published	Yes

Keywords

C. difficile
CDI
Diagnostic accuracy
LAMP
Sensitivity
Specificity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Lloyd, A., Pasupuleti, V., Thota, P., Pant, C., Rolston, D. D. K., Hernández, A. V., Benites Zapata, V., Fraser, T. G., Donskey, C. J., & Deshpande, A. (2015). Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: A systematic review. Diagnostic Microbiology and Infectious Disease, 82(1), 4-10. https://doi.org/10.1016/j.diagmicrobio.2015.02.007, https://doi.org/10.1016/j.diagmicrobio.2015.02.007

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abstract = "{\textcopyright} 2015 Elsevier Inc. Loop-mediated isothermal DNA amplification (LAMP) is currently used as standalone diagnostic test for C. difficile infection (CDI). We assessed the diagnostic accuracy of LAMP for the diagnosis of CDI. We searched 5 databases to identify studies that compared LAMP with culture cytotoxicity neutralization assay or anaerobic toxigenic culture (TC) of C. difficile. We used the random-effects model to calculate pooled sensitivities, specificities, diagnostic odds ratios, and their 95% confidence intervals (CIs). The search of the databases yielded 16 studies (6979 samples) that met inclusion criteria. When TC was used as the gold standard (6572 samples), bivariate analysis yielded a mean sensitivity of 0.95 (95% CI, 0.93-0.97; I2=67.4) and a mean specificity of 0.99 (95% CI, 0.96-1.00; I2=97.0). LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting CDI. The results should, however, be interpreted only in the presence of clinical suspicion and symptoms of CDI.",

keywords = "C. difficile, CDI, Diagnostic accuracy, LAMP, Sensitivity, Specificity",

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doi = "10.1016/j.diagmicrobio.2015.02.007",

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Lloyd, A, Pasupuleti, V, Thota, P, Pant, C, Rolston, DDK, Hernández, AV , Benites Zapata, V, Fraser, TG, Donskey, CJ & Deshpande, A 2015, 'Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: A systematic review', Diagnostic Microbiology and Infectious Disease, vol. 82, no. 1, pp. 4-10. https://doi.org/10.1016/j.diagmicrobio.2015.02.007, https://doi.org/10.1016/j.diagmicrobio.2015.02.007

TY - JOUR

T1 - Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection

T2 - A systematic review

AU - Lloyd, Aaron

AU - Pasupuleti, Vinay

AU - Thota, Priyaleela

AU - Pant, Chaitanya

AU - Rolston, David D.K.

AU - Hernández, Adrian V.

AU - Benites Zapata, Vicente

AU - Fraser, Thomas G.

AU - Donskey, Curtis J.

AU - Deshpande, Abhishek

PY - 2015/5/1

Y1 - 2015/5/1

N2 - © 2015 Elsevier Inc. Loop-mediated isothermal DNA amplification (LAMP) is currently used as standalone diagnostic test for C. difficile infection (CDI). We assessed the diagnostic accuracy of LAMP for the diagnosis of CDI. We searched 5 databases to identify studies that compared LAMP with culture cytotoxicity neutralization assay or anaerobic toxigenic culture (TC) of C. difficile. We used the random-effects model to calculate pooled sensitivities, specificities, diagnostic odds ratios, and their 95% confidence intervals (CIs). The search of the databases yielded 16 studies (6979 samples) that met inclusion criteria. When TC was used as the gold standard (6572 samples), bivariate analysis yielded a mean sensitivity of 0.95 (95% CI, 0.93-0.97; I2=67.4) and a mean specificity of 0.99 (95% CI, 0.96-1.00; I2=97.0). LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting CDI. The results should, however, be interpreted only in the presence of clinical suspicion and symptoms of CDI.

AB - © 2015 Elsevier Inc. Loop-mediated isothermal DNA amplification (LAMP) is currently used as standalone diagnostic test for C. difficile infection (CDI). We assessed the diagnostic accuracy of LAMP for the diagnosis of CDI. We searched 5 databases to identify studies that compared LAMP with culture cytotoxicity neutralization assay or anaerobic toxigenic culture (TC) of C. difficile. We used the random-effects model to calculate pooled sensitivities, specificities, diagnostic odds ratios, and their 95% confidence intervals (CIs). The search of the databases yielded 16 studies (6979 samples) that met inclusion criteria. When TC was used as the gold standard (6572 samples), bivariate analysis yielded a mean sensitivity of 0.95 (95% CI, 0.93-0.97; I2=67.4) and a mean specificity of 0.99 (95% CI, 0.96-1.00; I2=97.0). LAMP is a useful diagnostic tool with high sensitivity and specificity for detecting CDI. The results should, however, be interpreted only in the presence of clinical suspicion and symptoms of CDI.

KW - C. difficile

KW - CDI

KW - Diagnostic accuracy

KW - LAMP

KW - Sensitivity

KW - Specificity

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Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: A systematic review

Abstract

Keywords

UN SDGs

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